Pickering Laboratories specialize in manufacturing of cation-exchange columns for amino acid analysis. No other techniques, including reversed-phase chromatography, have been shown to match post-column ion-exchange methods in quantitation and reproducibility. Advantages of this method, such as absence of matrix interferences, are especially important in analysis of native samples.

Lithium columns and buffers systems have high selectivity and are perfect for physiological fluids and food analysis.



Reagent: Trione®
Reactor: 130 °C, 0.5 mL
Reagent Flow Rate: 0.3 mL/min


UV-Vis Detector: λ=570 nm for primary amino acids
                           λ=440 nm for secondary amino acids

Reagent: 300 mg of OPA, 2 g Thiofluor™, 3 mL of 30 %
                Brij® 35 solution in 950 mL of OD104
Reactor: 45 °C, 0.15 mL
Reagent Flow Rate: 0.3 mL/min


Fluorometer: λex 330 nm, λem 465 nm

The recommended gradient conditions are subject to change without notice. This may happen because of lotspecific changes in the columns, or improvements in the overall method.

The recommended gradient for the column will always be included in the column package, and it supersedes the information in this catalog. Use the program recommended on the column data sheet for the initial testing.

The column oven temperature programming gives additional flexibility when optimizing methods. Using a temperature gradient allows the user to improve separation, shorten analysis time and fine-tune the method for detecting compounds of interest.