POLYETHER ANTIBIOTICS ANALYSIS COLUMNS
Polyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid supplements and final feeds, are best quantified using HPLC with post-column derivatization. Because of selectivity of the post-column reaction, almost no sample clean-up is needed.
Flow Rate: 0.7 mL/min, column temperature
40 °C, injection volume 10 μL
Mobile Phase: 90:10 of Methanol / 5 % Acetic Acid
in water, isocratic
(NON-METALLIC POST-COLUMN DERIVATIZATION SYSTEM IS REQUIRED):
Reagent 1: Concentrated Sulfuric Acid / Methanol (4:96 v/v)
Reactor 1: Ambient, 0.1 mL
Reagent 2: 60 g of Vanillin (or p-dimethylaminobenzaldehyde)
in 950 mL of Methanol
Reactor 2: 90 °C, 1.4 mL
Reagents Flow Rate: 0.3 mL/min
NOTE: Using 2-reagent system for this application
greatly extends life of reagents.
One-reagent method and post-column derivatization
system are also available.
UV-Vis Detector: Vanillin λ=520 nm, DMAB λ=450 nm